Tween-80, Emulsifier T-80


Tween-80 is light yellow to amber oily viscose liquid, non-toxic. It is easily soluble in water, soluble in ethanol, vegetable oil, ethyl acetate, and methanol, and insoluble in mineral oil. HLB value is 15.0. Tween-80 molecular weight is 428.6.

The Tween-80 is a nonionic surfactant and emulsifier. This synthetic compound is a viscous, water-soluble yellow liquid.

Tween-80 is derived from polyethoxylated sorbitan and oleic acid. The hydrophilic groups in this compound are polyethers, also known as polyoxyethylene groups, which are polymers of ethylene oxide. In the nomenclature of polysorbates, the numeric designation following polysorbate refers to the lipophilic group, in this case, the oleic acid.

Tween 80 Solubility in Water

Tween-80 can be miscible with water in any ratio. This has nothing to do with the tipping point (commonly called the cloud point in the surfactant industry).

The reason may be that when water is added to Tween-80 (or Tween-80 is added to water) too quickly or even all at once. This causes a local Tween-80 concentration to be too high. When the water is less than Tween 80, Tween-80 is the continuous phase, and water is the dispersed phase. That is, the water is dispersed in Tween-80. When the amount of water increases gradually, there is a phase inversion process. At this time, the viscosity will suddenly increase and form a gel, which is the phenomenon of most non-ionic surfactants.

Solution: Add Tween-80 to the water, add slowly, stirring while adding, and the water temperature is about 50 degrees.

CAS No.:


Molecular Formula:


Structure Formula:

tween-80 structure formula


AppearanceAmber to light yellow oily liquid
Acid value2 mgKOH/g Max.
Saponification value45-55 mgKOH/g
Hydroxyl value65-80 mgKOH/g
Water3.0% Max.
Boiling point<100°C (1013 hPa)
Density1.07 g/cm3 (25°C)
Flash point<113°C
pH value5~7 (50 g/l, H₂O, 20°C)
Vapor pressure>1.33 hPa (20°C)
Viscosity kinematic300~500 mm2/s (25°C)


Tween-80 is used as an emulsifier, wetting agent, penetrating agent, diffusant, etc.

Tween-80 is used as an emulsifier in foods.

For example, in ice cream, Tween-80 is added up to 0.5% (v/v) concentration to make the ice cream smoother and easier to handle and increase its resistance to melting. In addition, adding this substance prevents milk proteins from completely coating the fat droplets. This allows them to join together in chains and nets, which hold air in the mixture, providing a firmer texture that holds its shape as the ice cream melts.

This T-80 emulsifier is used as a wetting agent in flavored mouth drops such as Ice Drops, helping to provide a spreading feeling to other ingredients like SD alcohol and mint flavor.

The World Health Organization has suggested acceptable daily intake limits of 0~25 mg of polyoxyethylene sorbitan esters per kg body weight.

Tween-80 is also used as a surfactant in soaps and cosmetics (including eyedrops) or as a solubilizer in mouthwash. However, the cosmetic grade of polysorbate-80 may have more impurities than the food grade.

T-80 emulsifier is an excipient used to stabilize aqueous formulations of medications for parenteral administration and used as an emulsifier in making the popular antiarrhythmic amiodarone. It is also used as an excipient in some European and Canadian influenza vaccines. Influenza vaccines contain 2.5 μg of Tween-80 per dose.

There is no difference between Polysorbat-80 and Tween-80.

Tween Emulsifier

Emulsifiers play an important role in the stability of emulsions. Tween emulsifiers can reduce the free energy of the interface and form a firm emulsified film to form a stable emulsion.

The emulsifier is adsorbed on the droplet’s surface, which can effectively reduce the surface tension. The Tween emulsifier is oriented around the droplets to form a film, reducing the oil-water interfacial tension. This can effectively prevent the accumulation of droplets. The more neatly the emulsifier is arranged on the surface of the droplets, the firmer the emulsified film and the more stable the emulsion.

Tween emulsifier occupies a very important position in the food industry. It can improve food quality, prevent food deterioration, extend its shelf life, and improve its taste and appearance.


Acute oral toxicity (LD50): 25000 mg/kg in mice.

In general, the study results of the polysorbates demonstrated no carcinogenicity and genotoxicity. In repeated-dose toxicity studies, diarrhea was observed as the major symptom of toxicity. Some studies, however, show conflicting findings.

In a 2-year dietary study of polysorbate-80 in rats, a higher incidence of adrenal medullary pheochromocytomas was found, mainly in male rats.

Polysorbate-80 is used as a solubilizing agent in IV infusions of the antiarrhythmic drug amiodarone. Rare case reports of liver toxicity have been published suggesting polysorbate-80 may contribute to liver toxicity with the IV formulation of amiodarone. The package labeling of amiodarone warns that polysorbate-80 is also known to leach DEHP (dioctyl phthalate) from PVC, and dosing recommendations must be followed closely.

It was found that maternal toxicities such as loose stool, suppression of weight gain, and reduced intake were seen, and poor lactation was noted in some studies of mother rats administered 7.5% solutions of polysorbates.


In 200KG iron drum/plastic drum.

metal drum 200l plastic drum

Tween 80 Storage:

Handle with care. Tween-80 should be stored in a cool, dry, and draughty place. The shelf life is two years. Then, Tween-80 can still be used if qualified after re-check.


Emulsifier T-80, Polysorbate-80, Poegasorb-80, Tween-80 chemical.


Contact us if you want to get the latest Tween-80 price and download Tween-80 SDS/MSDS.

You can find the difference between this product and Tween-20 — What Is the Difference between Tween-20 and Tween-80?


1. Are Tween-20 and Tween-80 interchangable?

Tween-20 and Tween-80 are not interchangeable. Tween 80 is made by polymerizing dehydrated sorbitol monooleate with ethylene oxide. Tween 20 is a mixture of polyoxyethylene dehydrated sorbitol monolaurate and a portion of polyoxyethylene bis-dehydrated sorbitol monolaurate. In addition, both tween-20 and tween-80 have different uses and properties.

2. Can Tween-80 be autoclaved?

It is perfectly fine. It can improve the liquid culture single colony. However, after sterilization, when the temperature is high, Tween-80 is turbid, but the liquid is clarified when it cools down.